Prevalence of amylase producing bacteria from the gut of Apis dorsata from central India
Article Sidebar
Main Article Content
Abstract
Apis dorsata is a wild honey bee as a social insect that represents its unique ability to produce honey by utilizing nectar of flowers with the help of unique gut bacterial community. The present study aimed to indentify the amylase positive gut bacteria in A. dorsata using 16S rRNA from the forest around Wardha, Maharashtra, India. More than 30 colonies obtained from gut sample indicated as amylase positive. The better amylase producer identified as Bacillus aryabhattai Accession number MW534731 which is reported for the first time in A. dorsata from this region.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) (https://creativecommons.org/licenses/by-nc-sa/4.0/)
References
Bankova, V.S., de Castro, S.L., & Marcucci, M.C. (2000).
Propolis: recent advances in chemistry and plant
origin. Apidologie, 31(1): 3-15.
Behal, A., Singh, J., Sharma, M.K., Puri, P., Batra, N., (2006).
Studied thermostable amylase producing Bacillus sp., Int.
J. Agri. Boil., 8: 80-83.
Burhan, A., Nisa, U., Gökhan, C., Ömer, C., Ashabil, A., &
Osman, G. (2003). Enzymatic properties of a novel
thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochemistry, 38(10): 1397-1403.
Engel, P., Kwong, W.K., Mc Frederick, Q., Anderson, K.E., Barribeau, S.M., Chandler, J.A., Cornman, R.S., Dainat, J., de Miranda, J.R., Doublet, V., Emery, O., Evans, J.D., Farinelli, L., Flenniken, M.L., Granberg, F., Grasis, J.A., Gauthier, L., Hayer, J., Koch, H., Kocher, S., Martinson, V.G., Moran, N., Munoz-Torres, M., Newton, I., Paxton, R.J., Powell, E., Sadd, B.M., Schmid-Hempel, P., Schmid-Hempel, R., Song, S.J., Schwarz, R.S., van Engelsdorp, D., & Dainat, B. (2016). The bee microbiome: impact on bee health and model for evolution and ecology of host-microbe interactions. mBio, 7(2): e0216415.
Kanmani, R., Vijayabaskar, P., & Jayalakshmi, S. (2011). Work on effect of incubation period on amylase activity, enzyme produce by Bacillus sp., World Appl. Sci. J., 12(11): 2120-2128.
Niode, N.J., ADJI, A., RIMBING, J., TULUNG, M., & Tallei, T.E. (2021). Composition and diversity of bacteria from giant Asian honeybee Apis dorsata gut. Biodiversitas J. Biolo. Diver., 22(2).
Ojha, S.K., Singh, P.K., Mishra, S., Pattnaik, R., Dixit, S., & Verma, S.K. (2020). Response surface methodology based optimization and scale-up production of amylase from a novel bacterial strain, Bacillus aryabhattai KIIT BE-1. Biotechnology Reports, 27: 500-506.
Rai, M.M., Gore, D.G., Rathod, M.K., & Khurad, A.M. (2013). Evidence of transovarial transmission of Bacillus subtilis in the silkworm, Bombyx mori L. J. Phar. Res., 7(4): 318-323.
Wang, M., Xu, H., Yin, G., Zhao, W., & He, S. (2014). Diversity of bacteria in the honey stomach of Apis cerana and Apis mellifera during the rape blooming period. Chinese J. App. Entomol., 51(6): 1567-1575.
Wang, M., Zhao, W. Z., Xu, H., Wang, Z. W., & He, S. Y. (2015). Bacillus in the guts of honey bees (Apis mellifera; Hymenoptera: Apidae) mediate changes in amylase values. Euro. J. Entomol., 112(4): 619.