Effect of Zinc and Phosphorus Applications in Soil and Temperature Variation on Growth and Production of Black Gram (Vigna mungo L.)

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Published: Jun 30, 2026
Keywords:
Zinc, Phosphorus fertilization, Pigments, Reproductive yield, Black gram

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Sadhana Devi
Department of Botany, University of Lucknow, Lucknow, UP, India
Sanjoli Tripathi
Department of Botany, University of Lucknow, Lucknow, UP, India
Ria Jaiswal
Department of Botany, University of Lucknow, Lucknow, UP, India
S N Pandey
Department of Botany, University of Lucknow, Lucknow, UP, India

Abstract

A clay pot experiment was conducted in glass top wire house to study the interactive effects of zinc and phosphorus application in soil on growth (length and dry weight), some biochemical constituents (pigments and protein contents and the activity of catalase and peroxidase) and production of fruits and seeds. Black gram (Vigna mungo L., Var. Haramoti) seeds were sown in clay pots field with alluvial soil (Texture- sandy loam, pH moderately alkaline and low organic matter content). Biochemical parameters were determined at day 65 of the growth in leaves of black gram. Before sowing treatments were made such as I- nil (control), II- ZnSO4 @25 mg/kg soil, III- Single Super Phosphate (SSP) @50 mg/kg soil, IV- ZnSO4 + SSP and V- ZnSO4 + SSP + low temperature (20oC) exposure for 4 hrs. up to the 25 days. Single application of zinc sulphate promoted all the parameters observed. Phosphorus application @50 mg/kg soil supported maximum plant growth such as dry weight by +54% but showed low value of pigments and protein content. Overall study showed the antagonistic effect of zinc and phosphorus fertilization on biochemical constituents and reproductive yield of black gram.

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Devi, S., Tripathi, S., Jaiswal, R., & Pandey, S. N. (2026). Effect of Zinc and Phosphorus Applications in Soil and Temperature Variation on Growth and Production of Black Gram (Vigna mungo L.). Journal of Applied Bioscience, 52(1), 82–86. Retrieved from https://9vom.in/journals/index.php/joab/article/view/1214
Issue
Vol. 52 No. 1 (2026): Volume 52; Issue 1 (2026)
Section
Original Research Articles
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References

Agarwala, S. C., & Sharma, C. P. (1979). Recognising micronutrient disorders of crop plants on the basis of visible symptoms and plant analysis. CABI Digital Library.

Ammara, H., Zulfiqar, A., Saleem, A., Sardar, R., Zulfiqar, U., Raza,

M. A. S., & Alqahtani, M. D. (2026). Zinc-regulated growth, chlorophyll, and antioxidant activity drive biofortification in rice varieties. BioMetals, 39(1), 375-388. https://doi. org/10.1007/s10534-025-00768-5

Banerjee, P., Mukherjee, B., Venugopalan, V. K., Nath, R., Chandran,

M. A. S., Dessoky, E. S., & Hossain, A. (2021). Thermal response of spring–summer-grown black gram (Vigna mungo L. Hepper) in Indian subtropics. Atmosphere, 12(11), 1489.

Bhardwaj, G., Brar, P. S., Sharma, U., & Kaushal, R. (2024).

Study on synergistic and antagonistic interactions of P and Zn fertilization on sweet pepper. Journal of Plant Nutrition, 47(17), 2930-2939.

Dang, K., Mu, J., Tian, H., Gao, D., Zhou, H., Guo, L., & Zhang,

Q. (2024). Zinc regulation of chlorophyll fluorescence and carbohydrate metabolism in saline-sodic stressed rice seedlings. BMC Plant Biology, 24(1), 464. https://doi. org/10.1186/s12870-024-05170-w

Dey, S., Chowardhara, B., Regon, P., Kar, S., Saha, B., & Panda,

S. K. (2022). Iron deficiency in blackgram (Vigna mungo L.): redox status and antioxidant activity. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 156(2), 411-426.

Fathi, A., Shiade, S. R. G., Saleem, A., Shohani, F., Fazeli, A., Riaz, A., & Rahimi, M. (2025). Reactive oxygen species (ROS) and antioxidant systems in enhancing plant resilience against abiotic stress. International Journal of Agronomy, (1), https:// doi.org/10.1155/ioa/8834883

Hu, D., Zhang, J., Yang, Y., Yu, D., Zhang, H., & Zhang, D. (2025). Molecular mechanisms underlying plant responses to low phosphate stress and potential applications in crop improvement. New Crops, 2, 100064. https://doi.org/10.1016/j. ncrops.2024.100064

Imran, M., Rehim, A., Sarwar, N., & Hussain, S. (2016). Zinc bioavailability in maize grains in response of phosphorous–zinc interaction. Journal of Plant Nutrition and Soil Science, 179 (1), 60-66.

Joshi, D., & Rathore, B. S. (2023). Production Technology of Black Gram. From seed to harvest: a comprehensive Kharif crop production, 72.

Khan, F., Siddique, A. B., Shabala, S., Zhou, M., & Zhao, C. (2023). Phosphorus Plays Key Roles in Regulating Plants’ Physiological Responses to Abiotic Stresses. Plants, 12(15), 2861. https://doi.org/10.3390/plants12152861

Khokhar, J. S., Broadley, M. R., & Ander, E. L. (2024). Soil zinc surveillance frameworks can inform human nutrition studies: opportunities in India. Frontiers in Soil Science, 4, https://doi. org/10.3389/fsoil.2024.1421652

Kiruthika, K., Hemalatha, M., Dhamodharan, P., & Tamilarasan, C. (2024). Impact of foliar micronutrients on growth and yield of blackgram: a review. Int J Res Agron, 7(12), 210-6.

MacDonald, G. K., Bennett, E. M., Potter, P. A., & Ramankutty, N.

(2011). Agronomic phosphorus imbalances across the world’s croplands. Proceedings of the National Academy of Sciences, 108(7), 3086-3091.

Murphy, J. A. M. E. S., & Riley, J. P. (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica chimica acta, 27, 31-36.

Madaan, I., Sharma, P., Singh, A. D., Dhiman, S., Kour, J., Kumar, P., & Bhardwaj, R. (2025). Zinc and plant hormones: an updated review. Zinc in Plants, 193-223. https://doi.org/10.1016/B978-0-323-91314-0.00016-8

Nath, S., Dey, S., Kundu, R., & Paul, S. (2024). Phosphate and zinc interaction in soil and plants: a reciprocal cross-talk. Plant Growth Regulation, 104(2), p.591-615. https://doi. org/10.1007/s10725-024-01201-6

Pandey, S. N. (2018). Biomolecular functions of micronutrients toward abiotic stress tolerance in plants. In Plant Nutrients and Abiotic Stress Tolerance. Singapore: Springer Singapore, p.153-170. https://doi.org/10.1007/978-981-10-9044-8_6

Pandey, S. N. (2020). Role of micronutrients in biochemical responses of crops under abiotic stresses. In Sustainable Agriculture in the Era of climate change. Cham: Springer International Publishing, p.93-112. https://doi.org/10.1007/978-3-030-45669-6_4

Poirier, Y., Jaskolowski, A., & Clúa, J. (2022). Phosphate acquisition and metabolism in plants. Current Biology, 32(12), R623-R629.

Sharma, C. P. (2006). Plant micronutrients. CRC Press, USA Shehzadi, N., Mahmood, A., Kaleem, M., Chishti, M. S., Bashir,

H., Hashem, A., & Ishtiaq, A. (2024). Zinc and nitrogen mediate the regulation of growth, leading to the upregulation of antioxidant aptitude, physio-biochemical traits, and yield in wheat plants. Scientific Reports, 14(1), 12897. https://doi. org/10.1038/s41598-024-63423-y

Sudhalakshmi, C., Krishnasamy, R., Rajarajan, A., & Meena,

S. (2014). Genotypic Divergence in Expression of Zinc-Deficiency Symptoms of Rice (Oryza sativa L.) in Sand Culture. Communications in soil science and plant analysis, 45(22), 2932-2948. https://doi.org/10.1080/00103624.2014.954720

Tripathi, S., & Dawson, J. (2023). Influence of phosphorus, sulphur and zinc levels on growth and yield of black gram (Vigna mungo L.). Int. J. Environ. Clim. Change, 13(5), 31-35.