Biogenic synthesis of green-nanoparticles uising ethanolic extract of Nostoc punctiforme (Kützing Ex Hariot) hariot and assessment of antibacterial activity
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Abstract
Objective: The aim of the study is to synthesize CuNP and AgNP from Nostoc punctiforme (Kützing ex Hariot) Hariot and evaluation of their antibacterial activity against Escherichia coli and Klebsiella pneumonia.
Methodology and Results: Copper and silver nanoparticles from ethanolic extract of 15 days old N. punctiforme. The synthesis of AgNP and CuNP was confirmed due to change in the colour of the solution from pale green to dark black and pale yellow to dark brick red, respectively. The absorption spectra of the synthesized nanoparticles at the UV range between 220-260nm showing stable productions of the nanoparticles. The antimicrobial activity of synthesized nanoparticles was assessed using E. coli and K. pneumoniae. The Copper nanoparticles show more antibacterial effect against K. pneumoniae in comparison to the silver nanoparticles, ethanolic extract of N. punctiforme and Streptomycin. Similarly in case of E. coli copper nanoparticle is more effective than that of silver nanoparticle but less than the positive control.
Conclusions and application of findings: From the results obtained, it could be concluded that the antimicrobial activity of both the synthesized nanoparticles were effective against E. coli and K. pneumoniae. The copper nanoparticles show more antibacterial effect against K. pneumoniae in comparison to the silver nanoparticles, ethanolic extract of N. punctiforme and Streptomycin. Similarly in case of E. coli copper nanoparticle is more effective than that of silver nanoparticle but less than the positive control.
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